SSPB Recombinant Protein (Staphylococcus aureus)

• Gene Aliases:

  Staphylococcal cysteine proteinase B; Staphylopain B.

• Accession Number:

  WP_001088793

• Reactivity:

  Staphylococcus aureus

• Target:

  Cysteine protease able to degrade elastin, fibrogen, fibronectin and kininogen. Exhibits a strong preference for substrates where arginine is preceded by a hydrophobic amino acid. Promotes detachment of primary human keratinocytes. Along with other extracellular proteases is involved in colonization and infection of human tissues (By similarity) .

• Type:

  Protein

• Source:

  Yeast

• Purification:

  Affinity purified using IMAC

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Format:

  Lyophilized// 20mM Tris-HCl, 0.5M NaCl, 6% Trehalose (pH 8.0)

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20° C/-80° C. Our default final concentration of glycerol is 50%. _x005F Repeated freezing and thawing is not recommended. Store working aliquots at 4° C for up to one week.

• Molecular Weight:

  22 kDa

• Protein Length:

  220-393aa

• NCBI Gene Symbol:

  SSPB

• Protein Name:

  Staphopain B

• Gene Name URL:

  SSPB

• CAS Number:

  9000-83-3

• Nucleotide Accession Number:

  NZ_LN626917