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PvdA Recombinant Protein

CAT: 0247-OPCA03405-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0247-OPCA03405-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
9000-83-3
Gene Name
L-ornithine N5-oxygenase
Gene Aliases
L-ornithine N (5) -hydroxylase; L-ornithine N (5) -oxygenase; L-ornithine N5-oxygenase; PA2386; Pyoverdin biosynthesis protein A.
Gene ID
882167
Accession Number
NP_251076.1
Reactivity
Pseudomonas aeruginosa
Target
Catalyzes the conversion of L-ornithine to N (5) -hydroxyornithine, the first step in the biosynthesis of all hydroxamate-containing siderophores, such as pyoverdin. Pyoverdin is a hydroxamate siderophore composed of a 6,7-dihydroxyquinoline-containing fluorescent chromophore joined to the N-terminus of a partly cyclic octapeptide (D-Ser-L-Arg-D-Ser-L-N (5) -OH-Orn-L-Lys-L-N (5) -OH-Orn-L-Thr-L-Thr in strain PAO1) . Specific for NADPH, which plays a role in stabilization of the C4a-hydroperoxyflavin intermediate.
Type
Protein
Source
E.coli
Sequence
MTQATATAVVHDLIGVGFGPSNIALAIALQERAQAQGALEVLFLDKQGDYRWHGNTLVSQSELQISFLKDLVSLRNPTSPYSFVNYLHKHDRLVDFINLGTFYPCRMEFNDYLRWVASHFQEQSRYGEEVLRIEPMLSAGQVEALRVISRNADGEELVRTTRALVVSPGGTPRIPQVFRALKGDGRVFHHSQYLEHMAKQPCSSGKPMKIAIIGGGQSAAEAFIDLNDSYPSVQADMILRASALKPADDSPFVNEVFAPKFTDLIYSREHAERERLLREYHNTNYSVVDTDLIERIYGVFYRQKVSGIPRHAFRCMTTVERATATAQGIELALRDAGSGELSVETYDAVILATGYERQLHRQLLEPLAEYLGDHEIGRDYRLQTDERCKVAIYAQGFSQASHGLSDTLLSVLPVRAEEISGSLYQHLKPGTAARALHEHALAS
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Format
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
-20°C or -80°C
Molecular Weight
65.5 kDa
Protein Length
Recombinant
NCBI Gene Symbol
PvdA
Host or Source
Pseudomonas aeruginosa
Protein Name
L-ornithine N (5) -monooxygenase
Gene Name URL
PvdA
Nucleotide Accession Number
NC_002516.2

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