APOBEC3A Recombinant Protein

• Gene Name:

  APOBEC3A and APOBEC3B deletion hybrid|apolipoprotein B mRNA editing enzyme catalytic subunit 3A

• Gene Aliases:

  A3A; APOBEC3A; apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A; ARP3; bK150C2.1; DNA dC->dU-editing enzyme APOBEC-3A; phorbolin-1; PHRBN; probable DNA dC->dU-editing enzyme APOBEC-3A.

• Gene ID:

  100913187|200315

• Accession Number:

  NP_001180218.1

• Reactivity:

  Homo sapiens|Human

• Target:

  DNA deaminase (cytidine deaminase) with restriction activity against viruses, foreign DNA and mobility of retrotransposons. Exhibits antiviral activity against adeno-associated virus (AAV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons. Selectively targets single-stranded DNA and can deaminate both methylcytosine and cytosine in foreign DNA. Can induce somatic hypermutation in the nuclear and mitochondrial DNA. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.

• Type:

  Protein

• Source:

  E.coli

• Purification:

  Affinity purified using IMAC

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Format:

  Lyophilized// 20mM Tris-HCl, 0.5M NaCl, 6% Trehalose (pH 8.0)

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20° C/-80° C. Our default final concentration of glycerol is 50%. _x005F Repeated freezing and thawing is not recommended. Store working aliquots at 4° C for up to one week.

• Molecular Weight:

  39 kDa

• Protein Length:

  Recombinant

• NCBI Gene Symbol:

  APOBEC3A|APOBEC3A_B

• Protein Name:

  DNA dC->dU-editing enzyme APOBEC-3A

• Gene Name URL:

  APOBEC3A|APOBEC3A_B

• CAS Number:

  9000-83-3

• Nucleotide Accession Number:

  NM_001193289.1