A2m Recombinant Protein

• Gene Name:

  Alpha-2-macroglobulin|alpha-2-macroglobulin-like

• Gene Aliases:

  A2m1; A2maa; A2MAC1; A2mb; alpha-2-M; alpha-2-macroglobulin; alpha-2-macroglobulin-P; Mam.

• Gene ID:

  100911545|24153

• Accession Number:

  NP_036620.2

• Reactivity:

  Rat|Rattus norvegicus

• Target:

  Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced) . Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

• Type:

  Protein

• Source:

  Yeast

• Sequence:

  LIYDLLPVKDLTGFPQGADQREEDTNGCVKQNDTYINGILYSPVQNTNEEDMYGFLKDMGLKVFTNSNIRKPKVCERLRDNKGIPAAYHLVSQSHMDAFLESSESPTETRRSYFPETWIWDLVVVDSAGVA

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Format:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  -20°C or -80°C

• Molecular Weight:

  16.9 kDa

• Protein Length:

  Recombinant

• NCBI Gene Symbol:

  A2m|LOC100911545

• Host or Source:

  Rat

• Protein Name:

  Alpha-2-macroglobulin

• Gene Name URL:

  A2m|LOC100911545

• CAS Number:

  9000-83-3

• Nucleotide Accession Number:

  NM_012488.2