ADH7 Recombinant Protein

• Gene Name:

  Alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide

• Gene Aliases:

  ADH4; alcohol dehydrogenase class 4 mu/sigma chain; alcohol dehydrogenase class IV mu/sigma chain; alcohol dehydrogenase VII; alcohol dehydrogenase-7; all-trans-retinol dehydrogenase [NAD (+) ] ADH7; class IV sigma-1 alcohol dehydrogenase; class IV sigmasigma alcohol dehydrogenase; gastric alcohol dehydrogenase; omega-hydroxydecanoate dehydrogenase ADH7; retinol dehydrogenase.

• Gene ID:

  131

• Accession Number:

  NP_000664.2

• Reactivity:

  Homo sapiens|Human

• Target:

  Could function in retinol oxidation for the synthesis of retinoic acid, a hormone important for cellular differentiation. Medium-chain (octanol) and aromatic (m-nitrobenzaldehyde) compounds are the best substrates. Ethanol is not a good substrate but at the high ethanol concentrations reached in the digestive tract, it plays a role in the ethanol oxidation and contributes to the first pass ethanol metabolism.

• Type:

  Protein

• Source:

  E.coli

• Purification:

  Affinity purified using IMAC

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Format:

  Lyophilized// 10mM Tris-HCl, 1mM EDTA, 6% Trehalose (pH 8.0)

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/ml. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20° C/-80° C. Our default final concentration of glycerol is 50%. Store working aliquots at 4° C for up to one week. Repeated freezing and thawing is not recommended.

• Molecular Weight:

  45.5 kDa

• Protein Length:

  Recombinant

• NCBI Gene Symbol:

  ADH7

• Host or Source:

  Human

• Protein Name:

  Alcohol dehydrogenase class 4 mu/sigma chain

• Gene Name URL:

  ADH7

• CAS Number:

  9000-83-3

• Nucleotide Accession Number:

  NM_000673.4