Recombinant human Steroid 21-hydroxylase

• Gene Name:

  Cytochrome P450 family 21 subfamily A member 2

• Gene Aliases:

  21-OHase; CA21H; CAH1; CPS1; CYP21; CYP21B; Cytochrome P450 21; cytochrome P450 XXI; cytochrome P450, family 21, subfamily A, polypeptide 2; cytochrome P450, subfamily XXIA (steroid 21-hydroxylase, congenital adrenal hyperplasia), polypeptide 2; Cytochrome P-450c21; Cytochrome P450-C21; cytochrome P450-C21B; P450c21B; steroid 21 hydroxylase; steroid 21-hydroxylase; steroid 21-monooxygenase.

• Gene ID:

  1589

• Reactivity:

  Homo sapiens|Human

• Target:

  A cytochrome P450 monooxygenase that plays a major role in adrenal steroidogenesis. Catalyzes the hydroxylation at C-21 of progesterone and 17alpha-hydroxyprogesterone to respectively form 11-deoxycorticosterone and 11-deoxycortisol, intermediate metabolites in the biosynthetic pathway of mineralocorticoids and glucocorticoids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) .

• Type:

  Protein

• Source:

  E.coli

• Purification:

  Affinity purified using IMAC

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Format:

  Lyophilized// 10mM Tris-HCl, 1mM EDTA, 6% Trehalose (pH 8.0)

• Molecular Weight:

  60 kDa

• Protein Length:

  Recombinant

• NCBI Gene Symbol:

  CYP21A2

• Protein Name:

  Steroid 21-hydroxylase

• Gene Name URL:

  CYP21A2

• CAS Number:

  9000-83-3