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NORMAL GOAT SERUM (NGS)

CAT: 0247-OORA01661-10MLSize: 10 mLDry Ice: NoHazardous: No
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CAT#:0247-OORA01661-10MLSize:10 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Gene Aliases
Blocking goat serum, blocking grade, 10% NGS
Target
Normal Goat Serum can be used as a blocking agent to treat plastic surfaces, membrane or tissue after they have been sensitized with primary antibody or antigen. It provides an alternative to bovine serum albumin (BSA) and non-fat dry milk. It is effective in reducing nonspecific binding of proteins to reaction surfaces, thereby maximizing signal-to-noise ratio. This blocking agent is recommended for use in immunoassays where the primary antibody was produced in goat, as a source of non-specific serum protein or on tissue for immunohistochemical applications.
Conjugation
Unconjugated
Type
Others
Applications
Chromatin Immunoprecipitation, Enzyme-linked immunosorbent assay, Flow cytometry, Fluorescence-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Sodium sodecyl sulfate - polyacrylamide gel electrophoresis, Western blot
Purification
Goat Serum blocking reagent or NGS was prepared from normal serum by a multi-step process which includes delipidation and selective precipitation. Assay by immunoelectrophoresis resulted in a multiple precipitin arcs against anti-Goat Serum. Normal Goat Serum was obtained from non-immunized healthy goats.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Concentration
105 mg/ml
Format
Lyophilized// 0.02M Potassium Phosphate, 0.15M Sodium Chloride (pH 7.2) with 0.01% (w/v) sodium azide and 0.01% (w/v) gentamicin sulfate
Reconstitution
Store Normal Goat Serum (NGS) at 4° C prior to restoration. Restore with 10 ml of deionized water (or equivalent) . For extended storage aliquot contents and freeze Goat Serum at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

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