STREPTAVIDIN

• Gene Aliases:

  SA, S avidin, streptococcus avidin

• Reactivity:

  Streptavidin, Streptomyces avidinii

• Target:

  Streptavidin

• Conjugation:

  Unconjugated

• Type:

  Others

• Applications:

  Chromatin Immunoprecipitation, Enzyme-linked immunosorbent assay, Gel shift assay, Immunofluorescence, Microarray, Sodium sodecyl sulfate - polyacrylamide gel electrophoresis, Western blot

• Purification:

  Streptavidin is chromatographically pure.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1.0 mg/mL by UV absorbance at 280 nm (prior to lyophilization)

• Bioactivity:

  15.5 U/mg by biotin titration method

• Format:

  Lyophilized

• Reconstitution:

  Restore with deionized water (or equivalent) and reconstitution volume is 5.0 mL. Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Streptavidin is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration is 1 year from date of opening.

• Specificity:

  Shows predominantly a single 53,600 dalton band by SDS-PAGE.

• Protein Name:

  P22629