ALS2 ELISA Kit (Human)

CAT: 0247-OKWB05427-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKWB05427-96WSize:96 Well
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Alsin Rho guanine nucleotide exchange factor
Gene Aliases
ALSJ, PLSJ, IAHSP, ALS2CR6
Gene ID
57679
Accession Number
NP_001129217
Reactivity
Human
Target
May act as a GTPase regulator. Controls survival and growth of spinal motoneurons.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology ALS2 ELISA Kit (Human) (OKWB05427) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for ALS2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ALS2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ALS2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.313-20 ng/mL
Sensitivity
0.188 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
ALS2
Protein Name
Alsin
Gene Name URL
ALS2
Nucleotide Accession Number
NM_020919

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