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KGF ELISA Kit (Rabbit)

CAT: 0247-OKWB00423-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKWB00423-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Reactivity
Oryctolagus cuniculus|Rabbit
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric, OD 450 nm
Assay Principle
Aviva Systems Biology Kgf ELISA Kit (Rabbit) (OKWB00423) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Kgf has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Kgf is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Kgf captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
31.25-2000pg/ml
Recovery
Sample Type Range Mean Serum (n=5) 86-104 92 EDTA Plasma (n=5) 85-102 91 Heparin Plasma (n=5) 86-94 91 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 86-104 92 Serum (n=5) 86-104 92 EDTA Plasma (n=5) 85-102 91 EDTA Plasma (n=5) 85-102 91 Heparin Plasma (n=5) 86-94 91 Heparin Plasma (n=5) 86-94 91
Sensitivity
18.75 pg/mL
Reconstitution
Store as indicated in product manual.
Components
Component Amount Kgf Microplate 96 Wells (12 x 8 Well strips) Kgf Lyophilized Standard 2 x 2 ng 100X Kgf Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount Kgf Microplate 96 Wells (12 x 8 Well strips) Kgf Microplate 96 Wells (12 x 8 Well strips) Kgf Lyophilized Standard 2 x 2 ng Kgf Lyophilized Standard 2 x 2 ng 100X Kgf Biotinylated Detector Anitbody 1 x 120 uL 100X Kgf Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL
NCBI Gene Symbol
KGF
Protein Name
Fibroblast growth factor
Gene Name URL
KGF

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