RAP1GAP2 ELISA Kit (Rabbit)

Mean Inter-Assay Precision

7732-18-5

100354130

XP_002718919

Rabbit

Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. Also required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet-activating factor (PAF) by removing the acetyl group at the SN-2 position.

ELISA Kit

Enzyme-linked Immunosorbent assay-Competitive

Colorimetric, OD 450 nm

Aviva Systems Biology Rap1gap2 ELISA Kit (Rabbit) (OKWB00414) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Rap1gap2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Rap1gap2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Rap1gap2 captured in the well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids

0.156-10ng/ml

Sample Type Range Mean Serum (n=5) 86-104 93 EDTA Plasma (n=5) 89-102 97 Heparin Plasma (n=5) 86-101 94 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 86-104 93 Serum (n=5) 86-104 93 EDTA Plasma (n=5) 89-102 97 EDTA Plasma (n=5) 89-102 97 Heparin Plasma (n=5) 86-101 94 Heparin Plasma (n=5) 86-101 94

0.938 ng/mL

Store as indicated in product manual.

Component Amount Rap1gap2 Microplate 96 Wells (12 x 8 Well strips) Rap1gap2 Lyophilized Standard 2 x 100 ng 100X Rap1gap2 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount Rap1gap2 Microplate 96 Wells (12 x 8 Well strips) Rap1gap2 Microplate 96 Wells (12 x 8 Well strips) Rap1gap2 Lyophilized Standard 2 x 100 ng Rap1gap2 Lyophilized Standard 2 x 100 ng 100X Rap1gap2 Biotinylated Detector Anitbody 1 x 120 uL 100X Rap1gap2 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL

RAP1GAP2

Platelet-activating factor acetylhydrolase IB subunit alpha

RAP1GAP2