Products for Research Use Only
RegisterLogin

APOB100 ELISA Kit (Rabbit)

CAT: 0247-OKWB00400-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKWB00400-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Reactivity
Oryctolagus cuniculus|Rabbit
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric, OD 450 nm
Assay Principle
Aviva Systems Biology Apob100 ELISA Kit (Rabbit) (OKWB00400) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Apob100 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Apob100 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Apob100 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
78.125-5000ng/ml
Recovery
Sample Type Range Mean Serum (n=5) 85-100 91 EDTA Plasma (n=5) 95-104 98 Heparin Plasma (n=5) 87-95 90 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 85-100 91 Serum (n=5) 85-100 91 EDTA Plasma (n=5) 95-104 98 EDTA Plasma (n=5) 95-104 98 Heparin Plasma (n=5) 87-95 90 Heparin Plasma (n=5) 87-95 90
Sensitivity
46.9 ng/mL
Reconstitution
Store as indicated in product manual.
Components
Component Amount Apob100 Microplate 96 Wells (12 x 8 Well strips) Apob100 Lyophilized Standard 2 x 5 ug 100X Apob100 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount Apob100 Microplate 96 Wells (12 x 8 Well strips) Apob100 Microplate 96 Wells (12 x 8 Well strips) Apob100 Lyophilized Standard 2 x 5 ug Apob100 Lyophilized Standard 2 x 5 ug 100X Apob100 Biotinylated Detector Anitbody 1 x 120 uL 100X Apob100 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL
NCBI Gene Symbol
APOB100
Protein Name
Apolipoprotein B-100
Gene Name URL
APOB100

Related Products

CatalogName
OKRC00166-96WOKRC00166-96W - ApoB100 ELISA Kit (Human)
OKCD06736-96WTTR ELISA Kit (Rabbit)
OKCA02208-96WTNNI3 ELISA Kit (Rabbit)
OKCD06572-96WAPOB ELISA Kit (Rabbit)
OKCD06515-96WSELP ELISA Kit (Rabbit)
OKCD01520-96WTIMP1 ELISA Kit (Rabbit)
OKCA00942-96WSFTPB ELISA Kit (Rabbit)
OKIA00128-96WIgM ELISA Kit (Rabbit)
OKCD05736-96WHGF ELISA Kit (Rabbit)
OKCD01486-96WTIMP3 ELISA Kit (Rabbit)

Alternative Products

CatalogName
GTR18806992-01Rabbit ApoB100 ELISA Kit
MBS2702129-01Rabbit Apolipoprotein B100 (APOB100) ELISA Kit
RK03390Rabbit Apolipoprotein B100 ELISA Kit (APOB100)
MBS8822490-01Rabbit APOB100 (Apolipoprotein B100) ELISA Kit
KLX0387GENLISA™ Rabbit Apolipoprotein B100 (APOB100) ELISA
B6187Apolipoprotein B100 (APOB100) Rabbit ELISA Kit
95233Apoprotein B100 (APOB100) Rabbit ELISA Kit
EK3F231-01Rabbit Apolipoprotein B100 (APOB100) ELISA Kit-Sandwich
EF016770ApoB100 ELISA Kit| Rabbit Apolipoprotein B100 ELISA Kit
517005Apolipoprotein B100 (APOB100) BioAssayTM ELISA Kit (Rabbit)

Popular Products