Products for Research Use Only
RegisterLogin

IFRD1 ELISA Kit (Rat)

CAT: 0247-OKWB00366-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKWB00366-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Gene Name
Interferon-related developmental regulator 1
Gene Aliases
Interferon-related developmental regulator 1; IRPR; nerve growth factor-inducible protein PC4; Pc4.
Gene ID
29596
Accession Number
NP_062115
Reactivity
Rat|Rattus norvegicus
Target
Probably participates in neurogenesis. Could play a role in regulating gene activity in the proliferative and/or differentiative pathways induced by NGF.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric, OD 450 nm
Assay Principle
Aviva Systems Biology Ifrd1 ELISA Kit (Rat) (OKWB00366) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ifrd1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ifrd1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ifrd1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
31.25-2000pg/ml
Recovery
Sample Type Range Mean Serum (n=5) 85-104 93 EDTA Plasma (n=5) 90-104 95 Heparin Plasma (n=5) 85-105 97 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 85-104 93 Serum (n=5) 85-104 93 EDTA Plasma (n=5) 90-104 95 EDTA Plasma (n=5) 90-104 95 Heparin Plasma (n=5) 85-105 97 Heparin Plasma (n=5) 85-105 97
Sensitivity
18.75 pg/mL
Reconstitution
Store as indicated in product manual.
Components
Component Amount Ifrd1 Microplate 96 Wells (12 x 8 Well strips) Ifrd1 Lyophilized Standard 2 x 2 ng 100X Ifrd1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount Ifrd1 Microplate 96 Wells (12 x 8 Well strips) Ifrd1 Microplate 96 Wells (12 x 8 Well strips) Ifrd1 Lyophilized Standard 2 x 2 ng Ifrd1 Lyophilized Standard 2 x 2 ng 100X Ifrd1 Biotinylated Detector Anitbody 1 x 120 uL 100X Ifrd1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL
NCBI Gene Symbol
Ifrd1
Protein Name
Interferon-related developmental regulator 1
Gene Name URL
Ifrd1
Nucleotide Accession Number
NM_019242

Related Products

CatalogName
OACA08516-50ULIFRD1 Antibody
OAAN03676-100ULIFRD1 Antibody
OAAN03676-50ULIFRD1 Antibody
OAAN03676-200ULIFRD1 Antibody
OAGA02325-100ULIFRD1 Antibody
OACA08516-100ULIFRD1 Antibody
OCOA16693-20UGIFRD1 Protein Lysate
OCOA14545-20UGIFRD1 Protein Lysate
AAP54585-100UGIFRD1 Peptide (AAP54585)
AAP54584-100UGIFRD1 Peptide (AAP54584)

Alternative Products

CatalogName
ELI-07905rRat Ifrd1 ELISA Kit
abx528113Rat Interferon-Related Developmental Regulator 1 (IFRD1) ELISA Kit
GTR10418570Rat Interferon related developmental regulator 1 (IFRD1) ELISA kit
E3525Interferon-Related developmental regulator 1 (IFRD1) Rat ELISA Kit
abx920257-01Rat IFRD1 siRNA
GTR19319940-01IFRD1 siRNA (Rat)
24283056IFRD1 Adenovirus (Rat)
RP205559IFRD1 Recombinant Protein (Rat)
abx985611-01Rat IFRD1 shRNA Plasmid
P42258PExpress-1-Ifrd1 (rat)

Popular Products