MAPK8IP1 ELISA Kit (Rat)

• Reproducibility:

  Mean Inter-Assay Precision

• Gene Name:

  Mitogen-activated protein kinase 8 interacting protein 1

• Gene Aliases:

  C-Jun-amino-terminal kinase-interacting protein 1; IB-1; islet-brain-1; Jip-1; JIP1; JIP-1-related protein; JNK MAP kinase scaffold protein 1; JNK-interacting protein 1; JRP; Mapk8ip; mitogen activated protein kinase 8 interacting protein; Mitogen-activated protein kinase 8-interacting protein 1.

• Gene ID:

  116457

• Accession Number:

  NP_446229

• Reactivity:

  Rat|Rattus norvegicus

• Target:

  The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Required for JNK activation in response to excitotoxic stress. Cytoplasmic MAPK8IP1 causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and thus inhibiting the JNK phosphorylation of c-Jun. May also participate in ApoER2-specific reelin signaling. Directly, or indirectly, regulates GLUT2 gene expression and beta-cell function. Appears to have a role in cell signaling in mature and developing nerve terminals. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. Functions as an anti-apoptotic protein and whose level seems to influence the beta-cell death or survival response (By similarity) .

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric, OD 450 nm

• Assay Principle:

  Aviva Systems Biology Mapk8ip1 ELISA Kit (Rat) (OKWB00352) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mapk8ip1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mapk8ip1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mapk8ip1 captured in the well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids

• Detection Range:

  1.563-100ng/ml

• Recovery:

  Sample Type Range Mean Serum (n=5) 87-103 95 EDTA Plasma (n=5) 86-102 93 Heparin Plasma (n=5) 91-104 96 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 87-103 95 Serum (n=5) 87-103 95 EDTA Plasma (n=5) 86-102 93 EDTA Plasma (n=5) 86-102 93 Heparin Plasma (n=5) 91-104 96 Heparin Plasma (n=5) 91-104 96

• Sensitivity:

  0.938 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Components:

  Component Amount Mapk8ip1 Microplate 96 Wells (12 x 8 Well strips) Mapk8ip1 Lyophilized Standard 2 x 100 ng 100X Mapk8ip1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount Mapk8ip1 Microplate 96 Wells (12 x 8 Well strips) Mapk8ip1 Microplate 96 Wells (12 x 8 Well strips) Mapk8ip1 Lyophilized Standard 2 x 100 ng Mapk8ip1 Lyophilized Standard 2 x 100 ng 100X Mapk8ip1 Biotinylated Detector Anitbody 1 x 120 uL 100X Mapk8ip1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL

• NCBI Gene Symbol:

  Mapk8ip1

• Protein Name:

  C-Jun-amino-terminal kinase-interacting protein 1

• Gene Name URL:

  Mapk8ip1

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_053777