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IL2 ELISA Kit (Monkey)

CAT: 0247-OKWB00303-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKWB00303-96WSize:96 Well
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Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Gene Name
Interleukin 2
Gene Aliases
IL-2; interleukin-2; T-cell growth factor; TCGF.
Gene ID
105731298
Accession Number
XP_007997918
Reactivity
Aotus nancymaae|Monkey
Target
Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG) . Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well. Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production. Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric, OD 450 nm
Assay Principle
Aviva Systems Biology IL2 ELISA Kit (Monkey) (OKWB00303) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
15.625-1000pg/ml
Recovery
Sample Type Range Mean Serum (n=5) 86-100 94 EDTA Plasma (n=5) 88-103 95 Heparin Plasma (n=5) 94-103 100 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 86-100 94 Serum (n=5) 86-100 94 EDTA Plasma (n=5) 88-103 95 EDTA Plasma (n=5) 88-103 95 Heparin Plasma (n=5) 94-103 100 Heparin Plasma (n=5) 94-103 100
Sensitivity
9.4 pg/mL
Reconstitution
Store as indicated in product manual.
Components
Component Amount IL2 Microplate 96 Wells (12 x 8 Well strips) IL2 Lyophilized Standard 2 x 1 ng 100X IL2 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount IL2 Microplate 96 Wells (12 x 8 Well strips) IL2 Microplate 96 Wells (12 x 8 Well strips) IL2 Lyophilized Standard 2 x 1 ng IL2 Lyophilized Standard 2 x 1 ng 100X IL2 Biotinylated Detector Anitbody 1 x 120 uL 100X IL2 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL
NCBI Gene Symbol
IL2
Protein Name
Interleukin-2
Gene Name URL
IL2
Nucleotide Accession Number
XM_007999727

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