MCPT4 ELISA Kit (Mouse)

• Reproducibility:

  Mean Inter-Assay Precision

• Gene Name:

  Mast cell protease 4

• Gene Aliases:

  Mast cell protease 4; Mcp; Mcp-; Mcp4; Mcp-4; MMCP-; MMCP-4; MMCP-4A; MMCP-4B; MSMCP; myo; myonase; serosal mast cell protease.

• Gene ID:

  17227

• Accession Number:

  NP_034909

• Reactivity:

  Mouse|Mus musculus

• Target:

  Has chymotrypsin-like activity. Hydrolyzes the amide bonds of synthetic substrates having Tyr and Phe residues at the P1 position. Preferentially hydrolyzes the 'Tyr-4-|-Ile-5' bond of angiotensin I and the 'Phe-20-|-Ala-21' bond of amyloid beta-protein, and is less active towards the 'Phe-8-|-His-9' bond of angiotensin I and the 'Phe-4-|-Ala-5' and 'Tyr-10-|-Glu-11' bonds of amyloid beta-protein. Involved in thrombin regulation and fibronectin processing.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric, OD 450 nm

• Assay Principle:

  Aviva Systems Biology Mcpt4 ELISA Kit (Mouse) (OKWB00279) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mcpt4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mcpt4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mcpt4 captured in the well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids

• Detection Range:

  46.875-3000pg/ml

• Recovery:

  Sample Type Range Mean Serum (n=5) 86-99 92 EDTA Plasma (n=5) 87-96 91 Heparin Plasma (n=5) 85-105 98 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 86-99 92 Serum (n=5) 86-99 92 EDTA Plasma (n=5) 87-96 91 EDTA Plasma (n=5) 87-96 91 Heparin Plasma (n=5) 85-105 98 Heparin Plasma (n=5) 85-105 98

• Sensitivity:

  28.1 pg/mL

• Reconstitution:

  Store as indicated in product manual.

• Components:

  Component Amount Mcpt4 Microplate 96 Wells (12 x 8 Well strips) Mcpt4 Lyophilized Standard 2 x 3 ng 100X Mcpt4 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount Mcpt4 Microplate 96 Wells (12 x 8 Well strips) Mcpt4 Microplate 96 Wells (12 x 8 Well strips) Mcpt4 Lyophilized Standard 2 x 3 ng Mcpt4 Lyophilized Standard 2 x 3 ng 100X Mcpt4 Biotinylated Detector Anitbody 1 x 120 uL 100X Mcpt4 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL

• NCBI Gene Symbol:

  Mcpt4

• Protein Name:

  Mast cell protease 4

• Gene Name URL:

  Mcpt4

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_010779