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RASAL1 ELISA Kit (Mouse)

CAT: 0247-OKWB00257-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKWB00257-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Gene Name
RAS protein activator like 1 (GAP1 like)
Gene Aliases
M; MRASAL; ras GTPase-activating-like protein; RAS protein activator like 1; rasGAP-activating-like protein 1.
Gene ID
19415
Accession Number
NP_038860
Reactivity
Mouse|Mus musculus
Target
Probable inhibitory regulator of the Ras-cyclic AMP pathway. Plays a role in dendrite formation by melanocytes.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric, OD 450 nm
Assay Principle
Aviva Systems Biology Rasal1 ELISA Kit (Mouse) (OKWB00257) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Rasal1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Rasal1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Rasal1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
78.125-5000pg/ml
Recovery
Sample Type Range Mean Serum (n=5) 85-103 93 EDTA Plasma (n=5) 85-97 92 Heparin Plasma (n=5) 87-103 98 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 85-103 93 Serum (n=5) 85-103 93 EDTA Plasma (n=5) 85-97 92 EDTA Plasma (n=5) 85-97 92 Heparin Plasma (n=5) 87-103 98 Heparin Plasma (n=5) 87-103 98
Sensitivity
46.9 pg/mL
Reconstitution
Store as indicated in product manual.
Components
Component Amount Rasal1 Microplate 96 Wells (12 x 8 Well strips) Rasal1 Lyophilized Standard 2 x 5 ng 100X Rasal1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount Rasal1 Microplate 96 Wells (12 x 8 Well strips) Rasal1 Microplate 96 Wells (12 x 8 Well strips) Rasal1 Lyophilized Standard 2 x 5 ng Rasal1 Lyophilized Standard 2 x 5 ng 100X Rasal1 Biotinylated Detector Anitbody 1 x 120 uL 100X Rasal1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL
NCBI Gene Symbol
Rasal1
Protein Name
RasGAP-activating-like protein 1
Gene Name URL
Rasal1
Nucleotide Accession Number
NM_013832

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