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H6PD ELISA Kit (Mouse)

CAT: 0247-OKWB00252-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKWB00252-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Gene Name
Hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)
Gene Aliases
AI785303; G6pd; G6pd1; GDH/6PGL endoplasmic bifunctional protein; glucose 1-dehydrogenase; glucose-6-phosphate dehydrogenase 1; Gpd; Gpd-; Gpd1; Gpd-1.
Gene ID
100198
Accession Number
NP_001277933
Reactivity
Mouse|Mus musculus
Target
Oxidizes glucose-6-phosphate and glucose, as well as other hexose-6-phosphates.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric, OD 450 nm
Assay Principle
Aviva Systems Biology H6pd ELISA Kit (Mouse) (OKWB00252) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for H6pd has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for H6pd is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample H6pd captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
0.156-10ng/ml
Recovery
Sample Type Range Mean Serum (n=5) 87-96 93 EDTA Plasma (n=5) 93-101 98 Heparin Plasma (n=5) 87-103 92 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 87-96 93 Serum (n=5) 87-96 93 EDTA Plasma (n=5) 93-101 98 EDTA Plasma (n=5) 93-101 98 Heparin Plasma (n=5) 87-103 92 Heparin Plasma (n=5) 87-103 92
Sensitivity
0.094 mU/mL
Reconstitution
Store as indicated in product manual.
Components
Component Amount H6pd Microplate 96 Wells (12 x 8 Well strips) H6pd Lyophilized Standard 2 x 10 U 100X H6pd Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount H6pd Microplate 96 Wells (12 x 8 Well strips) H6pd Microplate 96 Wells (12 x 8 Well strips) H6pd Lyophilized Standard 2 x 10 U H6pd Lyophilized Standard 2 x 10 U 100X H6pd Biotinylated Detector Anitbody 1 x 120 uL 100X H6pd Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL
NCBI Gene Symbol
H6pd
Protein Name
GDH/6PGL endoplasmic bifunctional protein
Gene Name URL
H6pd
Nucleotide Accession Number
NM_001291004

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