Products for Research Use Only
RegisterLogin

GABPB1 ELISA Kit (Human)

CAT: 0247-OKWB00232-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKWB00232-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Gene Name
GA binding protein transcription factor subunit beta 1
Gene Aliases
BABPB2; E4TF1; E4TF1-47; E4TF1-53; E4TF1B; GA binding protein transcription factor beta subunit 1 transcript variant gamma-2; GA-binding protein subunit beta-1; GABP subunit beta-2; GABPB; GABPB-1; GABPB2; NRF2B1; NRF2B2; nuclear respiratory factor 2; transcription factor E4TF1-47; transcription factor E4TF1-53.
Gene ID
2553
Accession Number
NP_001307839
Reactivity
Homo sapiens|Human
Target
Transcription factor capable of interacting with purine rich repeats (GA repeats) . Necessary for the expression of the Adenovirus E4 gene.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric, OD 450 nm
Assay Principle
Aviva Systems Biology GABPB1 ELISA Kit (Human) (OKWB00232) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GABPB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GABPB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GABPB1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
62.5-4000pg/ml
Recovery
Sample Type Range Mean Serum (n=5) 85-100 91 EDTA Plasma (n=5) 89-103 98 Heparin Plasma (n=5) 86-101 92 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 85-100 91 Serum (n=5) 85-100 91 EDTA Plasma (n=5) 89-103 98 EDTA Plasma (n=5) 89-103 98 Heparin Plasma (n=5) 86-101 92 Heparin Plasma (n=5) 86-101 92
Sensitivity
37.5 pg/mL
Reconstitution
Store as indicated in product manual.
Components
Component Amount GABPB1 Microplate 96 Wells (12 x 8 Well strips) GABPB1 Lyophilized Standard 2 x 4 ng 100X GABPB1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount GABPB1 Microplate 96 Wells (12 x 8 Well strips) GABPB1 Microplate 96 Wells (12 x 8 Well strips) GABPB1 Lyophilized Standard 2 x 4 ng GABPB1 Lyophilized Standard 2 x 4 ng 100X GABPB1 Biotinylated Detector Anitbody 1 x 120 uL 100X GABPB1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL
NCBI Gene Symbol
GABPB1
Protein Name
GA-binding protein subunit beta-1
Gene Name URL
GABPB1
Nucleotide Accession Number
NM_001320910

Related Products

CatalogName
OAGA00234-100ULGABPB1 antibody
OAAN02047-50ULGABPB1 Antibody
OACA08062-50ULGABPB1 Antibody
OAAN02047-100ULGABPB1 Antibody
OAAN02047-200ULGABPB1 Antibody
OACA08062-100ULGABPB1 Antibody
OCOA14317-20UGGABPB1 Protein Lysate
OCOA10125-20UGGABPB1 Protein Lysate
OCOA10126-20UGGABPB1 Protein Lysate
OCOA01474-20UGGABPB1 Protein Lysate

Alternative Products

CatalogName
D5663GA-binding protein subunit β-1 (GABPB1) Human ELISA Kit
MBS2907635-01Human GABPB1/GA-binding protein subunit beta-1 ELISA Kit
abx257701Human GA Binding Protein Subunit Beta 1 (GABPB1) ELISA Kit
MBS9332089-01Human GA-binding protein subunit beta-1, GABPB1 ELISA Kit
GTR18917933-01Human GABPB1 protein
21149051GABPB1 Adenovirus (Human)
P5661-01Recombinant Human GABPB1
CRH1691-01GABPB1 siRNA (Human)
abx917421-01Human GABPB1 siRNA
EF015007Mouse Gabpb1 ELISA Kit

Popular Products