SMPD3 ELISA Kit (Human)

CAT: 0247-OKWB00231-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKWB00231-96WSize:96 Well
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Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Gene Name
Sphingomyelin phosphodiesterase 3
Gene Aliases
Neutral sphingomyelinase 2; neutral sphingomyelinase II; nSMase-2; NSMASE2; sphingomyelin phosphodiesterase 3.
Gene ID
55512
Accession Number
NP_061137
Reactivity
Homo sapiens|Human
Target
Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric, OD 450 nm
Assay Principle
Aviva Systems Biology SMPD3 ELISA Kit (Human) (OKWB00231) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SMPD3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SMPD3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SMPD3 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
0.156-10ng/ml
Recovery
Sample Type Range Mean Serum (n=5) 87-103 96 EDTA Plasma (n=5) 86-102 95 Heparin Plasma (n=5) 87-100 94 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 87-103 96 Serum (n=5) 87-103 96 EDTA Plasma (n=5) 86-102 95 EDTA Plasma (n=5) 86-102 95 Heparin Plasma (n=5) 87-100 94 Heparin Plasma (n=5) 87-100 94
Sensitivity
0.094 ng/mL
Reconstitution
Store as indicated in product manual.
Components
Component Amount SMPD3 Microplate 96 Wells (12 x 8 Well strips) SMPD3 Lyophilized Standard 2 x 10 ng 100X SMPD3 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount SMPD3 Microplate 96 Wells (12 x 8 Well strips) SMPD3 Microplate 96 Wells (12 x 8 Well strips) SMPD3 Lyophilized Standard 2 x 10 ng SMPD3 Lyophilized Standard 2 x 10 ng 100X SMPD3 Biotinylated Detector Anitbody 1 x 120 uL 100X SMPD3 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL
NCBI Gene Symbol
SMPD3
Protein Name
Sphingomyelin phosphodiesterase 3
Gene Name URL
SMPD3
Nucleotide Accession Number
NM_018667

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