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SSC5D ELISA Kit (Human)

CAT: 0247-OKWB00228-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKWB00228-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Gene Name
Scavenger receptor cysteine rich family member with 5 domains
Gene Aliases
S5D-SRCRB; scavenger receptor cysteine rich domain containing (5 domains) ; scavenger receptor cysteine rich family, 5 domains; scavenger receptor cysteine-rich glycoprotein; soluble scavenger protein with 5 SRCR domains; soluble scavenger receptor cysteine-rich domain-containing protein SSC5D; soluble scavenger with 5 domains.
Gene ID
284297
Accession Number
NP_001138422
Reactivity
Homo sapiens|Human
Target
Binds to extracellular matrix proteins. Binds to pathogen-associated molecular patterns (PAMPs) present on the cell walls of Gram-positive and Gram-negative bacteria and fungi, behaving as a pattern recognition receptor (PRR) . Induces bacterial and fungal aggregation and subsequent inhibition of PAMP-induced cytokine release. Does not possess intrinsic bactericidal activity. May play a role in the innate defense and homeostasis of certain epithelial surfaces (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric, OD 450 nm
Assay Principle
Aviva Systems Biology SSC5D ELISA Kit (Human) (OKWB00228) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SSC5D has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SSC5D is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SSC5D captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
0.313-20ng/ml
Recovery
Sample Type Range Mean Serum (n=5) 85-104 95 EDTA Plasma (n=5) 88-105 97 Heparin Plasma (n=5) 87-105 97 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 85-104 95 Serum (n=5) 85-104 95 EDTA Plasma (n=5) 88-105 97 EDTA Plasma (n=5) 88-105 97 Heparin Plasma (n=5) 87-105 97 Heparin Plasma (n=5) 87-105 97
Sensitivity
0.188 ng/mL
Reconstitution
Store as indicated in product manual.
Components
Component Amount SSC5D Microplate 96 Wells (12 x 8 Well strips) SSC5D Lyophilized Standard 2 x 20 ng 100X SSC5D Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount SSC5D Microplate 96 Wells (12 x 8 Well strips) SSC5D Microplate 96 Wells (12 x 8 Well strips) SSC5D Lyophilized Standard 2 x 20 ng SSC5D Lyophilized Standard 2 x 20 ng 100X SSC5D Biotinylated Detector Anitbody 1 x 120 uL 100X SSC5D Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL
NCBI Gene Symbol
SSC5D
Protein Name
Soluble scavenger receptor cysteine-rich domain-containing protein SSC5D
Gene Name URL
SSC5D
Nucleotide Accession Number
NM_001144950

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