NBAS ELISA Kit (Human)

• Reproducibility:

  Mean Inter-Assay Precision

• Gene Name:

  Glucosidase II alpha subunit

• Gene Aliases:

  Alpha-glucosidase 2; G2AN; GIIA; Glucosidase II subunit alpha; GLUII; neutral alpha-glucosidase AB; PKD3.

• Gene ID:

  23193

• Accession Number:

  NP_056993

• Reactivity:

  Homo sapiens|Human

• Target:

  Catalytic subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc (2) Man (9) GlcNAc (2) oligosaccharide precursor of immature glycoproteins (PubMed:10929008) . Required for PKD1/Polycystin-1 and PKD2/Polycystin-2 maturation and localization to the cell surface and cilia (PubMed:27259053) .

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric, OD 450 nm

• Assay Principle:

  Aviva Systems Biology NBAS ELISA Kit (Human) (OKWB00226) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NBAS has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NBAS is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NBAS captured in the well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids

• Detection Range:

  0.781-50mIU/ml

• Recovery:

  Sample Type Range Mean Serum (n=5) 86-102 93 EDTA Plasma (n=5) 86-104 92 Heparin Plasma (n=5) 85-103 93 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 86-102 93 Serum (n=5) 86-102 93 EDTA Plasma (n=5) 86-104 92 EDTA Plasma (n=5) 86-104 92 Heparin Plasma (n=5) 85-103 93 Heparin Plasma (n=5) 85-103 93

• Sensitivity:

  0.469 mIU/mL

• Reconstitution:

  Store as indicated in product manual.

• Components:

  Component Amount NBAS Microplate 96 Wells (12 x 8 Well strips) NBAS Lyophilized Standard 2 x 50 U 100X NBAS Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount NBAS Microplate 96 Wells (12 x 8 Well strips) NBAS Microplate 96 Wells (12 x 8 Well strips) NBAS Lyophilized Standard 2 x 50 U NBAS Lyophilized Standard 2 x 50 U 100X NBAS Biotinylated Detector Anitbody 1 x 120 uL 100X NBAS Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL

• NCBI Gene Symbol:

  GANAB

• Protein Name:

  Neutral alpha-glucosidase AB

• Gene Name URL:

  GANAB

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_015909