CLPB ELISA Kit (Human)

Mean Inter-Assay Precision

7732-18-5

Caseinolytic mitochondrial matrix peptidase chaperone subunit B

ANKCLB; ankyrin-repeat containing bacterial clp fusion; caseinolytic peptidase B protein homolog; ClpB caseinolytic peptidase B homolog; ClpB homolog, mitochondrial AAA ATPase chaperonin; HSP78; MEGCANN; MGCA7; SKD3; suppressor of potassium transport defect 3; testicular secretory protein Li 11.

81570

NP_001245321

Homo sapiens|Human

May function as a regulatory ATPase and be related to secretion/protein trafficking process.

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Colorimetric, OD 450 nm

Aviva Systems Biology CLPB ELISA Kit (Human) (OKWB00210) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CLPB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CLPB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CLPB captured in the well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids

0.781-50ng/ml

Sample Type Range Mean Serum (n=5) 86-98 94 EDTA Plasma (n=5) 86-104 95 Heparin Plasma (n=5) 93-103 98 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 86-98 94 Serum (n=5) 86-98 94 EDTA Plasma (n=5) 86-104 95 EDTA Plasma (n=5) 86-104 95 Heparin Plasma (n=5) 93-103 98 Heparin Plasma (n=5) 93-103 98

0.469 ng/mL

Store as indicated in product manual.

Component Amount CLPB Microplate 96 Wells (12 x 8 Well strips) CLPB Lyophilized Standard 2 x 50 ng 100X CLPB Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount CLPB Microplate 96 Wells (12 x 8 Well strips) CLPB Microplate 96 Wells (12 x 8 Well strips) CLPB Lyophilized Standard 2 x 50 ng CLPB Lyophilized Standard 2 x 50 ng 100X CLPB Biotinylated Detector Anitbody 1 x 120 uL 100X CLPB Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL

CLPB

Caseinolytic peptidase B protein homolog

CLPB