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LAMTOR1 ELISA Kit (Human)

CAT: 0247-OKWB00202-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKWB00202-96WSize:96 Well
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Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Gene Name
Late endosomal/lysosomal adaptor, MAPK and MTOR activator 1
Gene Aliases
C11orf59; late endosomal/lysosomal adaptor and MAPK and MTOR activator 1; lipid raft adaptor protein p18; p18; p27kip1 releasing factor from RhoA; p27Kip1-releasing factor from RhoA; p27RF-Rho; PDRO; protein associated with DRMs and endosomes; ragulator complex protein LAMTOR1; ragulator complex protein PDRO; Ragulator1; RhoA activator C11orf59.
Gene ID
55004
Accession Number
NP_060377
Reactivity
Homo sapiens|Human
Target
As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. LAMTOR1 is directly responsible for anchoring the Ragulator complex to membranes. Also required for late endosomes/lysosomes biogenesis it may regulate both the recycling of receptors through endosomes and the MAPK signaling pathway through recruitment of some of its components to late endosomes. May be involved in cholesterol homeostasis regulating LDL uptake and cholesterol release from late endosomes/lysosomes. May also play a role in RHOA activation.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric, OD 450 nm
Assay Principle
Aviva Systems Biology LAMTOR1 ELISA Kit (Human) (OKWB00202) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LAMTOR1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LAMTOR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LAMTOR1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
0.313-20ng/ml
Recovery
Sample Type Range Mean Serum (n=5) 86-103 95 EDTA Plasma (n=5) 85-99 90 Heparin Plasma (n=5) 86-104 95 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 86-103 95 Serum (n=5) 86-103 95 EDTA Plasma (n=5) 85-99 90 EDTA Plasma (n=5) 85-99 90 Heparin Plasma (n=5) 86-104 95 Heparin Plasma (n=5) 86-104 95
Sensitivity
0.188 ng/mL
Reconstitution
Store as indicated in product manual.
Components
Component Amount LAMTOR1 Microplate 96 Wells (12 x 8 Well strips) LAMTOR1 Lyophilized Standard 2 x 20 ng 100X LAMTOR1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount LAMTOR1 Microplate 96 Wells (12 x 8 Well strips) LAMTOR1 Microplate 96 Wells (12 x 8 Well strips) LAMTOR1 Lyophilized Standard 2 x 20 ng LAMTOR1 Lyophilized Standard 2 x 20 ng 100X LAMTOR1 Biotinylated Detector Anitbody 1 x 120 uL 100X LAMTOR1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL
NCBI Gene Symbol
LAMTOR1
Protein Name
Ragulator complex protein LAMTOR1
Gene Name URL
LAMTOR1
Nucleotide Accession Number
NM_017907

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