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POTEC ELISA Kit (Human)

CAT: 0247-OKWB00171-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKWB00171-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Gene Name
POTE ankyrin domain family member C
Gene Aliases
A26B2; ANKRD26-like family B member 2; cancer/testis antigen family 104, member 6; CT104.6; POTE ankyrin domain family member C; POTE18; POTE-18; prostate, ovary, testis-expressed protein on chromosome 18.
Gene ID
388468
Accession Number
NP_001131143
Reactivity
Homo sapiens|Human
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric, OD 450 nm
Assay Principle
Aviva Systems Biology POTEC ELISA Kit (Human) (OKWB00171) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for POTEC has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for POTEC is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample POTEC captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
31.25-2000pg/ml
Recovery
Sample Type Range Mean Serum (n=5) 85-105 92 EDTA Plasma (n=5) 91-100 96 Heparin Plasma (n=5) 87-97 94 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 85-105 92 Serum (n=5) 85-105 92 EDTA Plasma (n=5) 91-100 96 EDTA Plasma (n=5) 91-100 96 Heparin Plasma (n=5) 87-97 94 Heparin Plasma (n=5) 87-97 94
Sensitivity
18.75 pg/mL
Reconstitution
Store as indicated in product manual.
Components
Component Amount POTEC Microplate 96 Wells (12 x 8 Well strips) POTEC Lyophilized Standard 2 x 2 ng 100X POTEC Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount POTEC Microplate 96 Wells (12 x 8 Well strips) POTEC Microplate 96 Wells (12 x 8 Well strips) POTEC Lyophilized Standard 2 x 2 ng POTEC Lyophilized Standard 2 x 2 ng 100X POTEC Biotinylated Detector Anitbody 1 x 120 uL 100X POTEC Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL
NCBI Gene Symbol
POTEC
Protein Name
POTE ankyrin domain family member C
Gene Name URL
POTEC
Nucleotide Accession Number
NM_001137671

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