CELA2B ELISA Kit (Human)

Mean Inter-Assay Precision

7732-18-5

Chymotrypsin like elastase 2B

Chymotrypsin like elastase family member 2B; chymotrypsin-like elastase family member 2B; ELA2B; elastase 2B; Elastase-2B; pancreatic elastase IIB (ELA2B) ; RP11-265F14.2.

51032

NP_056933

Homo sapiens|Human

Acts upon elastin.

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Colorimetric, OD 450 nm

Aviva Systems Biology CELA2B ELISA Kit (Human) (OKWB00170) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CELA2B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CELA2B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CELA2B captured in the well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids

0.313-20ng/ml

Sample Type Range Mean Serum (n=5) 85-96 90 EDTA Plasma (n=5) 96-103 100 Heparin Plasma (n=5) 85-99 94 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 85-96 90 Serum (n=5) 85-96 90 EDTA Plasma (n=5) 96-103 100 EDTA Plasma (n=5) 96-103 100 Heparin Plasma (n=5) 85-99 94 Heparin Plasma (n=5) 85-99 94

0.188 ng/mL

Store as indicated in product manual.

Component Amount CELA2B Microplate 96 Wells (12 x 8 Well strips) CELA2B Lyophilized Standard 2 x 20 ng 100X CELA2B Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount CELA2B Microplate 96 Wells (12 x 8 Well strips) CELA2B Microplate 96 Wells (12 x 8 Well strips) CELA2B Lyophilized Standard 2 x 20 ng CELA2B Lyophilized Standard 2 x 20 ng 100X CELA2B Biotinylated Detector Anitbody 1 x 120 uL 100X CELA2B Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL

CELA2B

Chymotrypsin-like elastase family member 2B

CELA2B