ORMDL2 ELISA Kit (Chicken)

• Reproducibility :

  Mean Inter-Assay Precision

• Gene Aliases :

  ORM1-like protein 2, RCJMB04_23j5

• Gene ID :

  425059

• Accession Number :

  NP_001026496

• Reactivity :

  Chicken

• Target :

  Negative regulator of sphingolipid synthesis.

• Type :

  ELISA Kit

• Assay Principle :

  Aviva Systems Biology Ormdl2 ELISA Kit (Chicken) (OKWB00128) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ormdl2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ormdl2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ormdl2 captured in the well.

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type :

  Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids

• Detection Range :

  1.563 - 100 ng/mL

• Recovery :

  Sample Type Range Mean Serum (n=5) 92-96 95 EDTA Plasma (n=5) 89-102 95 Heparin Plasma (n=5) 91-105 99 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 92-96 95 Serum (n=5) 92-96 95 EDTA Plasma (n=5) 89-102 95 EDTA Plasma (n=5) 89-102 95 Heparin Plasma (n=5) 91-105 99 Heparin Plasma (n=5) 91-105 99

• Sensitivity :

  0.938 ng/mL

• Reconstitution :

  Store as indicated in product manual.

• NCBI Gene Symbol :

  ORMDL2

• Protein Name :

  ORM1-like protein 2

• Gene Name URL :

  ORMDL2

• CAS Number :

  7732-18-5

• Nucleotide Accession Number :

  NM_001031325