Clusterin ELISA Kit (Rat)

7732-18-5

Clusterin

CLI, DAG, APOJ, SGP2, SP40, SGP-2, SP-40, Trpm2, Trpmb, TRPM-2, TRPM2B, RATTRPM2B

24854

Rat

Functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro) . Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. When secreted, protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Promotes apoptosis when in the nucleus. Inhibits apoptosis when associated with the mitochondrial membrane by interference with BAX-dependent release of cytochrome c into the cytoplasm. Plays a role in the regulation of cell proliferation.

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Colorimetric, OD450 nm

Aviva Systems Biology Clusterin ELISA Kit (Rat) (OKIA00138) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Clusterin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Clusterin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Clusterin captured in the well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, plasma and other biological fluids

12.5 ng/ml - 800 ng/ml

3.399 ng/ml

Rat

In Solution

Store as indicated in product manual.

Component Amount Anti-Rat Clusterin (Apolipoprotein J) Microplate 96 Wells (12 x 8 Well Strips) 20X Diluent Concentrate 50 mL 20X Wash Solution 50 mL 100X Enzyme-Antibody Conjugate 150 uL Chromogen-Substrate Solution 12 mL Stop Solution 12 mL Rat Clusterin (Apolipoprotein J) Calibrator 1 Component Amount Component Amount Anti-Rat Clusterin (Apolipoprotein J) Microplate 96 Wells (12 x 8 Well Strips) Anti-Rat Clusterin (Apolipoprotein J) Microplate 96 Wells (12 x 8 Well Strips) 20X Diluent Concentrate 50 mL 20X Diluent Concentrate 50 mL 20X Wash Solution 50 mL 20X Wash Solution 50 mL 100X Enzyme-Antibody Conjugate 150 uL 100X Enzyme-Antibody Conjugate 150 uL Chromogen-Substrate Solution 12 mL Chromogen-Substrate Solution 12 mL Stop Solution 12 mL Stop Solution 12 mL Rat Clusterin (Apolipoprotein J) Calibrator 1 Rat Clusterin (Apolipoprotein J) Calibrator 1

Formerly GWB-R374CL

CLU

Clusterin