AFAP1 ELISA Kit (Human)

• Reproducibility:

  Inter-Assay CV

• Gene Aliases:

  AFAP, AFAP110, AFAP-110

• Gene ID:

  60312

• Accession Number:

  NP_001128119

• Reactivity:

  Human

• Target:

  Can cross-link actin filaments into both network and bundle structures.

• Type:

  ELISA Kit

• Assay Principle:

  Aviva Systems Biology AFAP1 ELISA Kit (Human) (OKEI00131) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AFAP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AFAP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample AFAP1

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Biological Fluids

• Detection Range:

  0.781 - 50 ng/mL

• Sensitivity:

  0.469 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• NCBI Gene Symbol:

  AFAP1

• Protein Name:

  Actin filament-associated protein 1

• Gene Name URL:

  AFAP1

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001134647