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GP1BB ELISA Kit (Human)

CAT: 0247-OKEH08722-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH08722-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Glycoprotein Ib platelet subunit beta
Gene Aliases
Antigen CD42b-beta; BDPLT1; BS; CD42C; glycoprotein Ib (platelet), beta polypeptide; glycoprotein Ib platelet beta subunit; GP-Ib beta; GPIBB; GPIbbeta; nuclear localization signal deleted in velocardiofacial syndrome; platelet glycoprotein Ib beta chain; platelet membrane glycoprotein Ib beta; truncated platelet membrane glycoprotein Ib beta.
Gene ID
2812
Accession Number
NP_000398.1
Reactivity
Homo sapiens, Human
Target
Platelet glycoprotein Ib beta chain
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology GP1BB ELISA Kit (Human) (OKEH08722) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GP1BB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GP1BB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GP1BB captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103% (n = 20)
Sensitivity
9.4pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Platelet glycoprotein Ib beta chain
NCBI Gene Symbol
GP1BB
Protein Name
P13224
Gene Name URL
GP1BB
Nucleotide Accession Number
NM_000407.4

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