RAPH1 ELISA Kit (Human)

• Reproducibility:

  Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

• Gene Name:

  Ras association (RalGDS/AF-6) and pleckstrin homology domains 1

• Gene Aliases:

  ALS2CR18; ALS2CR9; amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 18; amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 9; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 18 protein; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 9 protein; lamellipodin; LPD; PREL2; PREL-2; proline-rich EVH1 ligand 2; Protein RMO1; RalGDS/AF-6; ras-associated and pleckstrin homology domains-containing protein 1; RMO1.

• Gene ID:

  65059

• Accession Number:

  NP_976241.1

• Reactivity:

  Homo sapiens, Human

• Target:

  Ras-associated and pleckstrin homology domains-containing protein 1

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology RAPH1 ELISA Kit (Human) (OKEH08548) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RAPH1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RAPH1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RAPH1 captured in well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Detection Range:

  78-5000pg/mL

• Recovery:

  Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 116% (n = 20)

• Sensitivity:

  38pg/mL

• Reconstitution:

  Store as indicated in product manual.

• Specificity:

  Natural and recombinant Human Ras-associated and pleckstrin homology domains-containing protein 1

• NCBI Gene Symbol:

  RAPH1

• Protein Name:

  Q70E73

• Gene Name URL:

  RAPH1

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_203365.3