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PARK7 ELISA Kit (Human)

CAT: 0247-OKEH08536-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH08536-96WSize:96 Tests
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Parkinsonism associated deglycase
Gene Aliases
DJ1; DJ-1; epididymis secretory sperm binding protein Li 67p; GATD2; HEL-S-67p; maillard deglycase; oncogene DJ1; Parkinson disease (autosomal recessive, early onset) 7; Parkinson disease protein 7; parkinson protein 7; Parkinsonism-associated deglycase; protein deglycase DJ-1; protein DJ-1; protein/nucleic acid deglycase DJ-1.
Gene ID
11315
Accession Number
NP_001116849.1
Reactivity
Homo sapiens, Human
Target
Protein/nucleic acid deglycase DJ-1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PARK7 ELISA Kit (Human) (OKEH08536) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PARK7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PARK7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PARK7 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111% (n = 20)
Sensitivity
18pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Protein/nucleic acid deglycase DJ-1
NCBI Gene Symbol
PARK7
Protein Name
Q99497
Gene Name URL
PARK7
Nucleotide Accession Number
NM_001123377.1

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