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SEMA6B ELISA Kit (Human)

CAT: 0247-OKEH08511-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH08511-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Semaphorin 6B
Gene Aliases
EPM11; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B; SEMAN; semaphorin VIB; semaphorin Z; semaphorin-6B; semaphorin-6Ba; Semaphorin-Z; SEMA-VIB; semaZ; SEM-SEMA-Y.
Gene ID
10501
Accession Number
NP_115484.2
Reactivity
Homo sapiens, Human
Target
Semaphorin-6B
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SEMA6B ELISA Kit (Human) (OKEH08511) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SEMA6B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SEMA6B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SEMA6B captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99% (n = 20)
Sensitivity
27pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Semaphorin-6B
NCBI Gene Symbol
SEMA6B
Protein Name
Q9H3T3
Gene Name URL
SEMA6B
Nucleotide Accession Number
NM_032108.3

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