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CASP4 ELISA Kit (Dog)

CAT: 0247-OKEH08390-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH08390-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Caspase 4, apoptosis-related cysteine peptidase
Gene Aliases
CASP1; CASP-1; caspase-1; caspase-1/caspase-4 hybrid; ICE; IL-1 beta-converting enzyme; IL-1BC; interleukin-1 beta convertase; interleukin-1 beta-converting enzyme; p45.
Gene ID
403724
Accession Number
NP_001003125.1
Reactivity
Canis lupus, Dog
Target
Caspase-1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CASP4 ELISA Kit (Dog) (OKEH08390) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CASP4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CASP4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CASP4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108% (n = 20)
Sensitivity
20pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Dog Caspase-1
NCBI Gene Symbol
CASP4
Protein Name
Q9MZV7
Gene Name URL
CASP4
Nucleotide Accession Number
NM_001003125.1

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