CLOCK ELISA Kit (Human)

• Reproducibility:

  Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

• Gene Name:

  Clock circadian regulator

• Gene Aliases:

  BHLHe8; circadian locomoter output cycles kaput protein; circadian locomoter output cycles protein kaput; class E basic helix-loop-helix protein 8; clock homolog; KAT13D.

• Gene ID:

  9575

• Accession Number:

  NP_001254772.1

• Reactivity:

  Homo sapiens, Human

• Target:

  Circadian locomoter output cycles protein kaput

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology CLOCK ELISA Kit (Human) (OKEH08376) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CLOCK has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CLOCK is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CLOCK captured in well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Detection Range:

  0.156-10ng/mL

• Recovery:

  Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 109% (n = 20)

• Sensitivity:

  0.089ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Specificity:

  Natural and recombinant Human Circadian locomoter output cycles protein kaput

• NCBI Gene Symbol:

  CLOCK

• Protein Name:

  O15516

• Gene Name URL:

  CLOCK

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001267843.1