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CNTNAP4 ELISA Kit (Human)

CAT: 0247-OKEH08340-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH08340-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Contactin associated protein family member 4
Gene Aliases
CASPR4; cell recognition molecule Caspr4; contactin associated protein like 4; contactin-associated protein-like 4.
Gene ID
85445
Accession Number
NP_207837.2
Reactivity
Homo sapiens, Human
Target
Contactin-associated protein-like 4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CNTNAP4 ELISA Kit (Human) (OKEH08340) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CNTNAP4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CNTNAP4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CNTNAP4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104% (n = 20)
Sensitivity
0.056ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Contactin-associated protein-like 4
NCBI Gene Symbol
CNTNAP4
Protein Name
Q9C0A0
Gene Name URL
CNTNAP4
Nucleotide Accession Number
NM_033401.4

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