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TDO2 ELISA Kit (Human)

CAT: 0247-OKEH08185-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH08185-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Tryptophan 2,3-dioxygenase
Gene Aliases
HYPTRP; TDO; TO; TPH2; TRPO; tryptamin 2,3-dioxygenase; tryptophan 2,3-dioxygenase; tryptophan oxygenase; tryptophan pyrrolase; tryptophanase.
Gene ID
6999
Accession Number
NP_005642.1
Reactivity
Homo sapiens, Human
Target
Tryptophan 2,3-dioxygenase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology TDO2 ELISA Kit (OKEH08185) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-TDO2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TDO2 and incubated. The TDO2 found in the sample or standards competes with the biotinylated TDO2 for limited binding sites on the immobilized anti-TDO2 antibody. Excess unbound biotinylated TDO2 and sample or standard TDO2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TDO2 captured in the well and inversely proportional to the amount of TDO2 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 83% (n = 20)
Sensitivity
0.29ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Tryptophan 2,3-dioxygenase
NCBI Gene Symbol
TDO2
Protein Name
P48775
Gene Name URL
TDO2
Nucleotide Accession Number
NM_005651.3

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