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NOD1 ELISA Kit (Mouse)

CAT: 0247-OKEH08040-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH08040-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Nucleotide-binding oligomerization domain containing 1
Gene Aliases
C230079P11; Car; Card4; caspase recruitment domain 4; caspase recruitment domain-containing protein 4; F830007N14Rik; Nl; Nlrc1; nucleotide-binding oligomerization domain-containing protein 1.
Gene ID
107607
Accession Number
NP_001164478.1
Reactivity
Mouse, Mus musculus
Target
Nucleotide-binding oligomerization domain-containing protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology NOD1 ELISA Kit (OKEH08040) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-NOD1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated NOD1 and incubated. The NOD1 found in the sample or standards competes with the biotinylated NOD1 for limited binding sites on the immobilized anti-NOD1 antibody. Excess unbound biotinylated NOD1 and sample or standard NOD1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated NOD1 captured in the well and inversely proportional to the amount of NOD1 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84% (n = 20)
Sensitivity
0.096ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Nucleotide-binding oligomerization domain-containing protein 1
NCBI Gene Symbol
Nod1
Protein Name
Q8BHB0
Gene Name URL
Nod1
Nucleotide Accession Number
NM_001171007.1

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