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CITED2 ELISA Kit (Mouse)

CAT: 0247-OKEH08007-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH08007-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2
Gene Aliases
AI835299; cbp/p300-interacting transactivator 2; ER154-like; melanocyte specific gene 2; Mrg; Mrg1; Msg; Msg2; MSG-related protein 1; p35; p35srj.
Gene ID
17684
Accession Number
NP_034958.2
Reactivity
Mouse, Mus musculus
Target
Cbp/p300-interacting transactivator 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CITED2 ELISA Kit (Mouse) (OKEH08007) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CITED2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CITED2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CITED2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89% (n = 20)
Sensitivity
0.054ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Cbp/p300-interacting transactivator 2
NCBI Gene Symbol
Cited2
Protein Name
O35740
Gene Name URL
Cited2
Nucleotide Accession Number
NM_010828.3

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