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CAPG ELISA Kit (Human)

CAT: 0247-OKEH07985-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH07985-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Capping actin protein, gelsolin like
Gene Aliases
Actin regulatory protein CAP-G; actin-regulatory protein CAP-G; AFCP; capping protein (actin filament), gelsolin-like; epididymis secretory protein Li 66; gelsolin-like capping protein; HEL-S-66; macrophage-capping protein; MCP.
Gene ID
822
Accession Number
NP_001243068.1
Reactivity
Homo sapiens, Human
Target
Macrophage-capping protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CAPG ELISA Kit (Human) (OKEH07985) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CAPG has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CAPG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CAPG captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94% (n = 20)
Sensitivity
0.089ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Macrophage-capping protein
NCBI Gene Symbol
CAPG
Protein Name
P40121
Gene Name URL
CAPG
Nucleotide Accession Number
NM_001256139.1

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