SRP14 ELISA Kit (Human)

CAT: 0247-OKEH07769-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH07769-96WSize:96 Tests
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Signal recognition particle 14
Gene Aliases
18 kDa Alu RNA-binding protein; ALURBP; homologous Alu RNA binding protein; signal recognition particle 14 kDa protein.
Gene ID
6727
Accession Number
NP_003125.3
Reactivity
Homo sapiens, Human
Target
Signal recognition particle 14 kDa protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SRP14 ELISA Kit (Human) (OKEH07769) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SRP14 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SRP14 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SRP14 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 119% (n = 20)
Sensitivity
0.049ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Signal recognition particle 14 kDa protein
NCBI Gene Symbol
SRP14
Protein Name
P37108
Gene Name URL
SRP14
Nucleotide Accession Number
NM_003134.5

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