CHEK1 ELISA Kit (Mouse)

• Reproducibility:

  Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

• Gene Name:

  Checkpoint kinase 1

• Gene Aliases:

  C85740; checkpoint kinase 1 homolog; Checkpoint kinase-1; Chk1; CHK1 checkpoint homolog; rad27; rad27 homolog; serine/threonine-protein kinase Chk1.

• Gene ID:

  12649

• Accession Number:

  NP_031717.2

• Reactivity:

  Mouse, Mus musculus

• Target:

  Serine/threonine-protein kinase Chk1

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology CHEK1 ELISA Kit (Mouse) (OKEH07727) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CHEK1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CHEK1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CHEK1 captured in well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Detection Range:

  0.156-10ng/mL

• Recovery:

  Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103% (n = 20)

• Sensitivity:

  0.056ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Specificity:

  Natural and recombinant Mouse Serine/threonine-protein kinase Chk1

• NCBI Gene Symbol:

  Chek1

• Protein Name:

  O35280

• Gene Name URL:

  Chek1

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_007691.5