TMLHE ELISA Kit (Human)

• Reproducibility:

  Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

• Gene Name:

  Trimethyllysine hydroxylase, epsilon

• Gene Aliases:

  AUTSX6; BBOX2; butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 2; epsilon-trimethyllysine 2-oxoglutarate dioxygenase; epsilon-trimethyllysine hydroxylase; TML hydroxylase; TML-alpha-ketoglutarate dioxygenase; TMLD; TMLH; TMLHED; trimethyllysine dioxygenase, mitochondrial; XAP130.

• Gene ID:

  55217

• Accession Number:

  NP_001171726.1

• Reactivity:

  Homo sapiens, Human

• Target:

  Trimethyllysine dioxygenase, mitochondrial

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Competitive

• Assay Principle:

  Aviva Systems Biology TMLHE ELISA Kit (OKEH07677) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-TMLHE antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TMLHE and incubated. The TMLHE found in the sample or standards competes with the biotinylated TMLHE for limited binding sites on the immobilized anti-TMLHE antibody. Excess unbound biotinylated TMLHE and sample or standard TMLHE is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TMLHE captured in the well and inversely proportional to the amount of TMLHE which was contained in the sample or standard.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Detection Range:

  0.625-40ng/mL

• Recovery:

  Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111% (n = 20)

• Sensitivity:

  0.306ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Specificity:

  Natural and recombinant Human Trimethyllysine dioxygenase, mitochondrial

• NCBI Gene Symbol:

  TMLHE

• Protein Name:

  Q9NVH6

• Gene Name URL:

  TMLHE

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001184797.1