TMLHE ELISA Kit (Human)

CAT: 0247-OKEH07677-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH07677-96WSize:96 Tests
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Trimethyllysine hydroxylase, epsilon
Gene Aliases
AUTSX6; BBOX2; butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetaine hydroxylase) 2; epsilon-trimethyllysine 2-oxoglutarate dioxygenase; epsilon-trimethyllysine hydroxylase; TML hydroxylase; TML-alpha-ketoglutarate dioxygenase; TMLD; TMLH; TMLHED; trimethyllysine dioxygenase, mitochondrial; XAP130.
Gene ID
55217
Accession Number
NP_001171726.1
Reactivity
Homo sapiens, Human
Target
Trimethyllysine dioxygenase, mitochondrial
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology TMLHE ELISA Kit (OKEH07677) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-TMLHE antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TMLHE and incubated. The TMLHE found in the sample or standards competes with the biotinylated TMLHE for limited binding sites on the immobilized anti-TMLHE antibody. Excess unbound biotinylated TMLHE and sample or standard TMLHE is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TMLHE captured in the well and inversely proportional to the amount of TMLHE which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
0.625-40ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111% (n = 20)
Sensitivity
0.306ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Trimethyllysine dioxygenase, mitochondrial
NCBI Gene Symbol
TMLHE
Protein Name
Q9NVH6
Gene Name URL
TMLHE
Nucleotide Accession Number
NM_001184797.1

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