DPP6 ELISA Kit (Human)

CAT: 0247-OKEH07625-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH07625-96WSize:96 Tests
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Dipeptidyl peptidase like 6
Gene Aliases
Dipeptidyl aminopeptidase IV-related protein; dipeptidyl aminopeptidase-like protein 6; Dipeptidyl aminopeptidase-related protein; Dipeptidyl peptidase 6; Dipeptidyl peptidase IV-like protein; dipeptidyl peptidase IV-related protein; dipeptidyl peptidase VI; dipeptidyl-peptidase 6; DPL1; DPP VI; DPPX; MRD33; VF2.
Gene ID
1804
Accession Number
NP_570629.2
Reactivity
Homo sapiens, Human
Target
Dipeptidyl aminopeptidase-like protein 6
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology DPP6 ELISA Kit (Human) (OKEH07625) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DPP6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DPP6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DPP6 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
1.56-100ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 114% (n = 20)
Sensitivity
0.77ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Dipeptidyl aminopeptidase-like protein 6
NCBI Gene Symbol
DPP6
Protein Name
P42658
Gene Name URL
DPP6
Nucleotide Accession Number
NM_130797.3

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