DFFB ELISA Kit (Human)

CAT: 0247-OKEH07623-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH07623-96WSize:96 Tests
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
DNA fragmentation factor subunit beta
Gene Aliases
CAD; caspase-activated deoxyribonuclease; caspase-activated DNase; caspase-activated nuclease; CPAN; DFF2; DFF40; DFF-40; DNA fragmentation factor 40 kDa subunit; DNA fragmentation factor subunit beta; DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) .
Gene ID
1677
Accession Number
NP_001307061.1
Reactivity
Homo sapiens, Human
Target
DNA fragmentation factor subunit beta
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology DFFB ELISA Kit (Human) (OKEH07623) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DFFB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DFFB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DFFB captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
125-8000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 110% (n = 20)
Sensitivity
62.5pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human DNA fragmentation factor subunit beta
NCBI Gene Symbol
DFFB
Protein Name
O76075
Gene Name URL
DFFB
Nucleotide Accession Number
NM_001320132.1

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