APOO ELISA Kit (Human)

CAT: 0247-OKEH07608-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH07608-96WSize:96 Well
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Apolipoprotein O
Gene Aliases
Apolipoprotein O; brain my025; FAM121B; family with sequence similarity 121B; Mic23; MIC26; MICOS complex subunit MIC23; MICOS complex subunit MIC26; MICOS26; mitochondrial contact site and cristae organizing system subunit 26; My025; Protein FAM121B.
Gene ID
79135
Accession Number
NP_077027.1
Reactivity
Homo sapiens, Human
Target
MICOS complex subunit MIC26
Type
ELISA Kit
Applications
Enzyme-linked immunosorbent assay
Assay Principle
Aviva Systems Biology APOO ELISA Kit (Human) (OKEH07608) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for APOO has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for APOO is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample APOO captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
78 - 5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84% (n = 20)
Sensitivity
30pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Apolipoprotein O
NCBI Gene Symbol
APOO
Protein Name
Q9BUR5
Gene Name URL
APOO
Nucleotide Accession Number
NM_024122.4

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