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HTR2C ELISA Kit (Human)

CAT: 0247-OKEH07576-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH07576-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
5-hydroxytryptamine receptor 2C
Gene Aliases
5-HT1C;5-HT2C;5HTR2C;5-HTR2C;5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled;5-hydroxytryptamine receptor 1C;5-hydroxytryptamine receptor 2C; HTR1C; serotonin 5-HT-1C receptor; serotonin 5-HT-2C receptor; Serotonin receptor 2C.
Gene ID
3358
Accession Number
NP_000859.1
Reactivity
Homo sapiens, Human
Target
5-hydroxytryptamine receptor 2C
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology HTR2C ELISA Kit (Human) (OKEH07576) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HTR2C has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HTR2C is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HTR2C captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 81% (n = 20)
Sensitivity
35pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human 5-hydroxytryptamine receptor 2C
NCBI Gene Symbol
HTR2C
Protein Name
P28335
Gene Name URL
HTR2C
Nucleotide Accession Number
NM_000868.3

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