Products for Research Use Only
RegisterLogin

CD40LG ELISA Kit (Pig)

CAT: 0247-OKEH07310-96WSize: 96 TestsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKEH07310-96WSize:96 Tests
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
CD40 ligand
Gene Aliases
CD40 ligand; CD40 ligand (TNF superfamily, member 5, hyper-IgM syndrome) ; CD40L; CD40-L; TNFSF5; tumor necrosis factor (ligand) superfamily, member 5; Tumor necrosis factor ligand superfamily member 5.
Gene ID
397231
Accession Number
NP_999291.1
Reactivity
Porcine, Sus scrofa
Target
CD40 ligand
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology CD40LG ELISA Kit (OKEH07310) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-CD40LG antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CD40LG and incubated. The CD40LG found in the sample or standards competes with the biotinylated CD40LG for limited binding sites on the immobilized anti-CD40LG antibody. Excess unbound biotinylated CD40LG and sample or standard CD40LG is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CD40LG captured in the well and inversely proportional to the amount of CD40LG which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 109% (n = 20)
Sensitivity
0.092ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Pig CD40 ligand
NCBI Gene Symbol
CD40LG
Protein Name
Q95MQ5
Gene Name URL
CD40LG
Nucleotide Accession Number
NM_214126.1

Related Products

CatalogName
OKCD05996-96WCD40LG ELISA Kit (Mouse)
OKEH07308-96WCD40LG ELISA Kit (Chicken)
OKEH03985-96WCD40LG ELISA Kit (Mouse)
OKCD00148-96WCD40LG ELISA Kit (Human)
OKEH07309-96WCD40LG ELISA Kit (Dog)
OKEH03809-96WCD40LG ELISA Kit (Bovine)
OKEH06893-96WCD40LG ELISA Kit (Rat)
OKCD02325-96WCD40LG ELISA Kit (Rat)
OKEH00201-96WCD40LG ELISA Kit (Human)
OKEH07308CD40LG ELISA Kit (Chicken) (OKEH07308)

Alternative Products

CatalogName
E0034PiPig CD40LG/CD40 ligand ELISA Kit
RK15063Pig CD40 ligand (CD40LG) ELISA Kit
abx154902-01Pig Cluster of Differentiation 40 Ligand / TNFSF5 (CD40LG) ELISA Kit
MBS1387500-01Recombinant Pig CD40 ligand (CD40LG), partial
R0119pPig CD40LG / CD40 ligand Recombinant Protein
ELA-E0119hHuman CD40LG ELISA Kit
GTR18818525-01Rat CD40LG ELISA Kit
GTR18817698-01Mouse CD40LG ELISA Kit
abx065938-01Pig Cluster of Differentiation 40 Ligand / TNFSF5 (CD40LG) Protein
abx491060-01Pig Cluster of Differentiation 40 Ligand / TNFSF5 (CD40LG) CLIA Kit

Popular Products