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CCL4 ELISA Kit (Pig)

CAT: 0247-OKEH07286-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH07286-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
C-C motif chemokine ligand 4
Gene Aliases
C-C motif chemokine 4; chemokine (C-C motif) ligand 4; macrophage inflammatory protein 1-beta; MIP1b; MIP-1-beta; MIP-1BETA; SCY4A; small-inducible cytokine A4.
Gene ID
396668
Accession Number
NP_998944.1
Reactivity
Porcine, Sus scrofa
Target
C-C motif chemokine 4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CCL4 ELISA Kit (Pig) (OKEH07286) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CCL4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CCL4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CCL4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104% (n = 20)
Sensitivity
7.8pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Pig C-C motif chemokine 4
NCBI Gene Symbol
CCL4
Protein Name
Q711P4
Gene Name URL
CCL4
Nucleotide Accession Number
NM_213779.1

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