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IFNA1 ELISA Kit (Chicken)

CAT: 0247-OKEH07238-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH07238-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Interferon, interferon type A1/A2-like
Gene Aliases
IFN alpha; IFNA; IFNA1; IFNA2; IFNA6; IFNAL4; IFNAL5; IFNAL6; IFN-alpha; IFN-gamma; interferon alpha; interferon type A1/A2; interferon type A1/A2-like 4; interferon type A1/A2-like 5; interferon type A1/A2-like 6; interferon type A3.
Gene ID
100857744, 100857947, 100858177, 100859032, 396398
Accession Number
NP_990758.1
Reactivity
Chicken, Gallus gallus
Target
Interferon type A1/A2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology IFNA1, IFNA3 ELISA Kit (Chicken) (OKEH07238) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IFNA1, IFNA3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IFNA1, IFNA3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IFNA1, IFNA3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 83% (n = 20)
Sensitivity
11.8pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Chicken Interferon type A1/A2
NCBI Gene Symbol
IFNA3, LOC100857744, LOC100857947, LOC100858177, LOC100859032
Protein Name
P42165
Gene Name URL
IFNA3, LOC100857744, LOC100857947, LOC100858177, LOC100859032
Nucleotide Accession Number
NM_205427.1

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